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Unlockable Stages
These unlockable stages will replace the ? marks on the Story menu.

Mona's Games - Beat Wario's stage
Jimmy's Games - Beat Mona's stage
Kat's and Ana's Games - Beat Jimmy's stage
Jimmy's Folk's Remix - Beat Kat's and Ana's stage
Dribble's and Spitz's Games - Beat Jimmy's Folk's remix
Dr. Crygor's Games - Beat Dribble's and Spitz's stage
WarioWatch Remix - Beat Dr. Crygor's stage
Orbulon's Games - Beat WarioWatch remix
9-Volt's and 18-Volt's Games - Beat Orbulon's stage
Warioman's Games - Beat 9-Volt's and 18-Volt's stage
Speedier Spin Remix - Get at least 50 points on WarioWatch
Skyscraper Remix - Beat Warioman's Games
Tower Remix - Get at least 25 points on Skyscraper
Mansion Remix - Get at least 25 points on Tower

Unlock WarioWare Twisted
When you complete every game in the spindex, you unlock WarioWare Twisted in the games section of souveniers. The game restarts to the GBA load screen, but the load screen looks different.

Minigame Towers
Once you complete Warioman you will unlock the Minigame Towers. I dont know if there is a boss tower.

Easy Tower - Complete Warioman
Hard Tower - Get 25 points on easy tower
Thrilling Tower - Get 25 points on hard tower

Unlock Mawaru Made in Wario "Minigame"
Get a high score on every microgame through 'Zukan' mode

Unlock Random Item
Complete a character's stage

Unlock Alien (Staff Credits) Item
Complete Wario's Final Stage

Unlock Pyoro R
Complete every single microgame at least once